Alternative Description: HNUP153
Catalogue Number: IQ178
Also known as: Nup153; 153 kDa nucleoporin; N153; Nuclear pore complex protein hnup153; Nuclear pore complex protein Nup153; Nucleoporin 153kDa; Nucleoporin Nup153;
Quantity: 2ml
Price: €328.00Price excludes VAT and Delivery.
Clone: N/A;
Host/Isotype: Sheep;
Immunogen: Synthetic peptide from a region of Human Nup153 (residues 1456-1475)
Specificity: Detects recombinant and endogenous Nup153 in human, rat & Xenopus
Purification: Affinity purified;
Format: Affinity purified antiserum containing PBS + 0.1% sodium azide;
Applications: IB; IF; IP;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline use neat for immunofluorescence & 1:10 for western blot
Relevance: Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex specific glycoproteins characterized by cytoplasmically oriented O linked N acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. Nup153 has three distinct domains: a N terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C terminal region containing multiple XFXFG repeats. Nup153 is a possible DNA binding subunit of the nuclear pore complex (NPC). The repeat containing domain may be involved in anchoring components of the pore complex to the pore membrane.
Research areas: Cell Biology; Nuclear Import / Export; Nucleus; Subcellular Markers; Tags & Cell Markers;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
