Alternative Description: Bromodeoxyuridine (BrdU)
Catalogue Number: IQ390
Description: Rat Anti-Bromodeoxyuridine (BrdU)
Quantity: 25ug
Price: £40.00Price excludes VAT and Delivery.
Clone: BU1/75 (ICR1);
Host/Isotype: Rat IgG2a;
Specificity: Reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. The antibody detects nuleated cells in S-phase which have had BrdU incorporated into their DNA. It also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not cross react with thymidine
Purification: Protein G purified;
Format: Purified antibody in PBS 50% Glycerol + 0.1% Sodium Azide;
Applications: ICC;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration but typical titrations for Immunocytochemistry are 1:200-1:500
Relevance: The immunocytochemical detection of Bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis
References: (click to view full details)
β-catenin promotes self-renewal of skeletal-muscle satellite cells- Ana Perez-Ruiz et al ( Journal of Cell Science ,April 2008)
Cux2 (Cutl2) integrates neural progenitor development with cell-cycle progression during spinal cord neurogenesis- Iulianella, A et al ( Development,Feb 2008)
Research areas: Cell Proliferation;
