Alternative Description: LAP2α
Catalogue Number: IQ175
Also known as: Thymopoietin; TP; TMPO;
Quantity: 0.1ml
Price: $365.00Price excludes VAT and Delivery.
Clone: N/A;
Host/Isotype: Rabbit;
Immunogen: Recombinant LAP2α C terminus (amino acids 188-693)
Specificity: Reacts with human, rat & mouse LAP2α
Purification: Unpurified;
Format: Antiserum containing PBS + 0.1% sodium azide;
Applications: IF; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline use up to 1:2500 for western blot & 1:250 for immunofluorescence
Relevance: Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eu karyotes such as the inner nuclear membrane proteins, Lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
References: (click to view full details)
The inner nuclear membrane protein Sun1 mediates the anchorage of Nesprin-2 to the nuclear envelope - V.C. Padmakumar et al ( Journal of Cell Science,August 3, 2005)
Thymopoietin (lamina-associated polypeptide 2) gene mutation associated with dilated cardiomyopathy- Matthew R.G. Taylor et al ( Human Mutation,24 Oct 2005)
Wild-Type Levels of Human Immunodeficiency Virus Type 1 Infectivity in the Absence of Cellular Emerin Protein- Ming-Chieh Shun et al ( Journal of Virology,January 2007)
Research areas: Cell Biology; Class V Lamins; Cytoskeleton; Cytoskeleton / ECM; Intermediate Filaments; Lamins;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
