Alternative Description: Nesprin 1
Catalogue Number: IQ171
Also known as: 8B; CPG2; Enaptin; MYNE1; Myocyte Nuclear Envelope Protein 1; Nesprin 1 Alpha; Nuclear Envelope Spectrin Repeat Protein 1; Spectrin Repeat Containing Nuclear Envelope 1; SYNE1;
Quantity: 0.1ml
Price: $365.00Price excludes VAT and Delivery.
Clone: N/A;
Host/Isotype: Rabbit;
Immunogen: Synthetic polypeptide GRSTPNRQKTPRGKC residues 3212-3226)conjugated to KLH
Specificity: Reacts with human Nesprin 1
Purification: Affinity purified;
Format: Affinity purified antiserum containing PBS + 0.1% sodium azide;
Applications: IB; IHC (frozen sections);
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration. However as a guideline try 1:500-1:750 for western blot, & 1:25-1:50 for immunofluorescence
Relevance: There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus.These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as "dystrophins of the nucleus" to maintain nuclear organization and structural integrity. There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus.These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as "dystrophins of the nucleus" to maintain nuclear organization and structural integrity.
Research areas: Cell Biology; Cytoskeleton; Cytoskeleton / ECM; Intermediate Filaments; Nuclear Envelope; Nucleus; Subcellular Markers; Tags & Cell Markers;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Western Blotting Protocol;
