Alternative Description: Ran
Catalogue Number: IQ172
Also known as: Androgen receptor associated protein 24; ARA 24; Gsp1; GTP binding nuclear protein RAN; GTPase Ran; LPS; RAN member RAS oncogene family; Ras like protein TC4; Ras related nuclear protein; RASL2 8;
Quantity: 0.2ml
Price: £219.00Price excludes VAT and Delivery.
Clone: N/A;
Host/Isotype: Rabbit;
Immunogen: His-tagged recombinant human Ran protein purified from E. coli
Specificity: Reacts with human & Xenopus Ran protein
Purification: Unpurified;
Format: Antiserum containing PBS + 0.1% sodium azide;
Applications: IB; IF; IP;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration however as guideline use up to 1/500 for western blot
Relevance: Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of Regulator of chromosome condensation 1 (RCC1). Mutations in Ran disrupt DNA synthesis. Because of its many functions, it is likely that Ran interacts with several other proteins. Ran regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. Ran could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of Ran-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Ran is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). Ran coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.
Research areas: Cell Biology; Cell Division; Co-activators/co-repressors; Nuclear Import / Export; Ras Family; signal transduction; Spindle;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
