Alternative Description: RanGAP
Catalogue Number: IQ173
Also known as: Fug 1; KIAA1835; MGC20266; OTTHUMP00000028918; RAN GTPase-activating protein 1; SD; Segregation distorter homolog;
Quantity: 0.2ml
Price: £328.00Price excludes VAT and Delivery.
Clone: N/A;
Host/Isotype: Rabbit;
Immunogen: A mixture of two peptides from Xenopus RanGAP, n-CHWSDMFTGRLRPEI-c & n-CPSPEKLVRMGPRRSA-c
Specificity: Reacts with human & Xenopus RanGAP
Purification: Unpurified;
Format: Antiserum containing PBS + 0.1% sodium azide;
Applications: IF; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration however as a guideline dilute up to 1/500 for western blot
Relevance: RanGAP1 is a GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.
Research areas: Cell Biology; Cell Division; Centromere;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Western Blotting Protocol;
