Alternative Description: RanGEF (RCC1)
Catalogue Number: IQ174
Also known as: Cell cycle regulatory protein; CHC 1; Chromosome condensation; Guanine nucleotide releasing; RCC 1; Regulator of chromosome condensation;
Quantity: 0.2ml
Price: £328.00Price excludes VAT and Delivery.
Clone: N/A;
Host/Isotype: Rabbit;
Immunogen: His-tagged recombinant Xenopus RCC1 protein purified from E. coli
Specificity: Reacts with human & Xenopus RanGEF
Purification: Unpurified;
Format: Antiserum containing PBS + 0.1% sodium azide;
Applications: IF; IP; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration however as a guideline dilute up to 1/500 for western blot
Relevance: Ran GTPase plays important roles in nucleocytoplasmic transport in interphase and in both spindle formation and nuclear envelope (NE) assembly during mitosis. The latter functions rely on the presence of high local concentrations of GTP bound Ran near mitotic chromatin. RanGTP localization has been proposed to result from the association of Ran's GDP/GTP exchange factor, RCC1, with chromatin , but Ran is shown here to bind directly to chromatin in two modes, either dependent or independent of RCC1, and, where bound, to increase the affinity of chromatin for NE membranes.
Research areas: Cell Biology; Cell Division; Other cell division antibodies; Spindle;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
