Alternative Description: Serine Protease Inhibitor 2A (Spi2A)
Catalogue Number: IQ318
Also known as: Serpin 2A; Serpin A3G; Serpina3g;
Quantity: 0.1ml (1mg/ml)
Price: £365.00Price excludes VAT and Delivery.
Clone: MoFo29.2;
Host/Isotype: Hamster IgG2b;
Immunogen: Peptide conjugated to KLH: 406-426 NPERSTNFPNGEGASSQR( C )
Myeloma/fusion partners: SP2/0;
Specificity: Mouse Spi2A
Purification: Protein G;
Format: Purified antibody containing PBS + 0.1% sodium azide;
Applications: IB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline try 1:1000
Relevance: Spi2A is a serine and cysteine protease inhibitor. It can inhibit lysosomal papain-like proteases including the cathepsins B, G, H, K, L and V. It is ineffective against elastase, granzyme A, granzyme B, or caspases 3, 8 or 9. Inhibition of cytoplasmic cathepsin B following release from the lysosome may protect cells from apoptosis. This may facilitate the survival of progenitor T-cells and the subsequent development of long term memory CD8 T-cells.
Research areas: Cell Biology; SERPINs;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
