Alternative Description: Nestin
Catalogue Number: IQ300
Also known as: ESTM 46; FLJ21841; Nbla00170; Nes;
Quantity: 0.1 ml
Price: £295.00Price excludes VAT and Delivery.
Clone: 2C1.3A11;
Host/Isotype: Mouse IgG1;
Immunogen: A 150-amino-acid fragment from the cloned human Nestin conjugated to GST
Specificity: Human Nestin with no cross- reactivity to rodent
Purification: Protein G;
Format: Purified antibody containing PBS + 0.1% sodium azide;
Applications: FACS; IB; ICC; IHC; IP;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline try at 1:1000-1:5000 for western blot & 1:100-1:500 for immuncytochemistry. Additional antigen retrieval may be necessary depending on fixation method.
Relevance: Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. This highly specific antibody to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.
References: (click to view full details)
Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody.- Messam CA ( Exp Neurol,Feb 2000)
Research areas: Angiogenic Factors; Intermediate Filaments; Intracellular; Neuron Restricted Lineage; Neuronal Markers; Neuroscience;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
