Alternative Description: hnRNP-L
Catalogue Number: IQ212
Also known as: D830027H13Rik; FLJ35509; hnRNPL; hnRPL; P/OKcl.14;
Quantity: 0.1ml (1mg/ml)
Price: $365.00Price excludes VAT and Delivery.
Clone: 4D11;
Host/Isotype: Mouse IgG1;
Immunogen: Human HNRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose
Myeloma/fusion partners: SP2/0;
Specificity: Human hnRNP-L. Detects a band of approximately 65 kDa. It cross reacts with mouse & newt
Purification: Protein A;
Format: Purified antibody (from supernatant) containing PBS + 0.1% sodium azide;
Applications: E; IF; IP; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline try 1:5000 for western blot
Relevance: Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (HNRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with HNRNP complexes and along with other HNRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of HNRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions.Strucutrally, hnRPL contains 2 segments of approximately 80 amino acids each which are weakly related to each other and to the ribonucleoprotein consensus sequence-type RNA-binding domains of other HNRNP and snRNP proteins.
References: (click to view full details)
A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts- Piņol-Roma S, Swanson MS, Gall JG, Dreyfuss G ( The Journal of Cell Biology,1989)
Research areas: Nucleoplasm; Nucleus; RNA binding; Subcellular Markers; Tags & Cell Markers;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
