Alternative Description: hnRNP-A2/B1
Catalogue Number: IQ215
Also known as: hnRNP A2; hnRNP A2 / hnRNP B1; hnRNP B1; hnRNP-A2; hnRNP-B1; RNP A2; RNP B1; RNP-A2; RNP-B1; RNPA2;
Quantity: 0.1ml (1mg/ml)
Price: £365.00Price excludes VAT and Delivery.
Clone: DP3B3;
Host/Isotype: Mouse IgG2a;
Immunogen: Bacterially expressed His-hnRNP-A2 fusion protein (Human)
Myeloma/fusion partners: SP2/0;
Specificity: Human (& chicken) hnRNP-A2/B1. Cross reacts with mouse for IHC on lung tissue. Reacts predominantly with a 36 kDa band (A2), & a 38kDa band (B1)
Purification: Protein A;
Format: Purified antibody (from supernatant) containing PBS + 0.1% sodium azide;
Applications: E; IF; IHC; IP; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline try 1:500 for western blot
Relevance: This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The HNRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.
References: (click to view full details)
β-Catenin Interacts With the FUS Proto-oncogene Product and Regulates Pre-mRNA Splicing - Satoshi Sato et al ( Gastroenterology,October 2005)
Altered expression of splicing factor, heterogeneous nuclear ribonucleoprotein A2/B1, in mouse lung neoplasia- Katherine A. Peebles et al ( Molecular Carcinogenesis,3 May 2007)
Molecular characterization of the hnRNP A2/B1 proteins: tissue-specific expression and novel isoforms.- Kamma H ( Exp Cell Res.,Feb 1999)
Protein composition of the intranuclear inclusions of FXTAS - C. K. Iwahashi et al ( Brain,October 24, 2005)
Research areas: RNA binding; RNA Processing;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
