Alternative Description: Mad1L1
Catalogue Number: IQ200
Also known as: hMAD1; HsMAD1; MAD1; MAD1L1; Mitotic arrest deficient 1 yeast homolog like 1; PIG 9; Tax binding protein 181; TP53I9; Tumor protein p53 inducible protein 9; TXBP181;
Quantity: 0.1ml (2mg/ml)
Price: €328.00Price excludes VAT and Delivery.
Clone: 9B10;
Host/Isotype: Mouse IgG2b;
Immunogen: Recombinant full length MAD1 protein (human)
Specificity: Human MAD1
Purification: Protein A;
Format: Purified antibody containing PBS + 0.1% sodium azide;
Applications: IF; IP;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline try 1:500 to 1:1000 for immunofluorescence, & 5μg/0.5-1ml for immunoprecipitation.
Relevance: Mad1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. Mad1L1 functions as a homodimer and interacts with Mad2L1. Mad1L1 may play a role in cell cycle control and tumor suppression.
References: (click to view full details)
Centromere-associated protein-E is essential for the mammalian mitotic checkpoint to prevent aneuploidy due to single chromosome loss- Weaver BA ( J Cell Biol,Aug 2003)
Research areas: HLH / Leucine Zipper; Transcription; Tumor Suppressors;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
