Alternative Description: hnRNP-A1
Catalogue Number: IQ206
Also known as: Helix destabilizing protein; hnRNP core protein A1; HNRNPA1; HNRPA1; MGC102835; Syncrip; Heterogeneous nuclear ribonucleoprotein A1; Heterogeneous nuclear ribonucleoprotein A1B; Heterogeneous nuclear ribonucleoprotein B2; Nuclear ribonucleoprotein particle A1; Single strand DNA binding protein; Single strand RNA binding protein;
Quantity: 0.1ml
Price: €328.00Price excludes VAT and Delivery.
Clone: 9H10;
Host/Isotype: Mouse IgG2b;
Immunogen: Full length hnRNP-A1 native protein (partially purified) from HeLa cells (human)
Myeloma/fusion partners: Balb/c & Sp2/0 cell line;
Specificity: Human hnRNP-A1. Detects a band of approximately 34 kDa
Purification: Protein A;
Format: Purified antibody (from supernatant) containing PBS + 0.1% sodium azide;
Applications: E; IF; IP; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline use 1:2000-1:4000 in western blot
Relevance: This protein belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The HNRNP proteins have distinct nucleic acid binding properties. HNRNP A1 has two repeats of quasi RRM domains that bind to RNAs. It is one of the most abundant core proteins of HNRNP complexes and it is localized to the nucleoplasm. This protein, along with other HNRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre mRNA into HNRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought to have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for the HNRNP A1 gene but only two transcripts are fully described. These variant have multiple alternative transcription initiation sites and multiple polyA sites.
References: (click to view full details)
hnRNP A1 functions with specificity in repression of SMN2 exon 7 splicing- Tsuyoshi Kashima ( Human Molecular Genetics,September 19, 2007)
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.- Burd CG ( Embo J,Mar 1994)
Research areas: RNA binding; RNA Processing;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
