Alternative Description: hnRNP-U
Catalogue Number: IQ210
Also known as: NRNPU; hnRNPU; HNRPU; p120; p120 nuclear protein; pp120; SAF-A; SAFA; Scaffold attachment factor A; U21.1;
Quantity: 0.1ml
Price: £328.00Price excludes VAT and Delivery.
Clone: 3G6;
Host/Isotype: Mouse IgG1;
Immunogen: RNP’s eluted from an oligo (dt) cellulose column
Myeloma/fusion partners: SP2/0;
Specificity: Human hnRNP-U. Detects a band of approximately 120 kDa. It cross reacts with monkey
Purification: Protein A;
Format: Purified antibody (from supernatant) containing PBS + 0.1% sodium azide;
Applications: E; IF; IP; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration
Relevance: The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The HNRNP proteins have distinct nucleic acid binding properties. hnRNP U is thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way.
References: (click to view full details)
Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies.- Dreyfuss G et al ( Molecular and Cellular Biology,Jun 1984)
Involvement of SUMO Modification in MBD1- and MCAF1-mediated Heterochromatin Formation- Yasuhiro Uchimura et al ( J. Biol. Chem,August 11, 2006)
Research areas: RNA binding; RNA Processing;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
