Alternative Description: Mad2L1
Catalogue Number: IQ286
Also known as: Homolog of MAD2 S cerevisiae; HsMAD2; Human mitotic feedback control protein Madp2; MAD 2; MAD 2A; MAD2 L1; MAD2 like protein 1; MAD2A; Mitotic arrest deficient yeast homolog like 1; Mitotic spindle assembly checkpoint protein;
Quantity: 50ul
Price: $295.00Price excludes VAT and Delivery.
Clone: 17D10;
Host/Isotype: Mouse IgG1;
Immunogen: Recombinant human MAD2
Specificity: Western blot with a variety of human cell lines confirms that the antibody is specific for Mad2L1. Cross species specificity has not yet been determined. Detects a band of approximately 25 kDa
Purification: Protein A;
Format: Purified antibody (from supernatant) containing PBS + 0.1% sodium azide;
Applications: IP; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however suggested working concentration for westerns is ~2μg/ml, for ip ~5μg/ml.
Relevance: Mad2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
References: (click to view full details)
Spindle proteins Aurora A and BUB1B, but not Mad2, are aberrantly expressed in dysplastic mucosa of patients with longstanding ulcerative colitis- Espen Burum-Auensen et al ( J Clin Pathol,February 2007)
Subcellular Localization of the Spindle Proteins Aurora A, Mad2, and BUBR1 Assessed by Immunohistochemistry - Espen Burum-Auensen et al ( Journal of Histochemistry and Cytochemistry,January 22, 2007)
Research areas: Cell Biology; Cell Division; Nucleus; Spindle; Subcellular Markers; Tags & Cell Markers;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
