Alternative Description: HEF-1/ Cas-L
Catalogue Number: IQ298
Also known as: CASL; dJ49G10.2; dJ761I2.1; HEF 1; NEDD9; NEDD9 protein; NY REN 12 antigen; p105; Protein NEDD9;
Quantity: 50μl
Price: €265.00Price excludes VAT and Delivery.
Clone: 14A11;
Host/Isotype: Mouse IgG2b kappa;
Immunogen: GST-HEF-1 (82-398aa.)
Myeloma/fusion partners: SP2/0;
Specificity: Specific for human HEF1 localized in mitotic cells in the spindle and the centrosome. It does not recognize p130Cas. Have not tested it on Sin1.
Purification: Protein G;
Format: Purified antibody containing PBS + 0.1% sodium azide;
Applications: IF; IP;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline try at 1μg of antibody per 200μg of whole cell lysate for IP, & 1:100 for IF
Relevance: HEF1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases; two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.
References: (click to view full details)
Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics.- O'Neill GM ( Molecular and Cellular Biology,Aug 2001)
Research areas: Apoptosis; Cell Biology; FAK Family; Intracellular; signal transduction; Transcription;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
