Alternative Description: PABPL1
Catalogue Number: IQ216
Also known as: PABP; PAB 1; PABPC 1; PABPC2; Poly A binding protein 1; Poly A binding protein cytoplasmic 1; Polyadenylate binding protein 1;
Quantity: 0.1ml (1mg/ml)
Price: $365.00Price excludes VAT and Delivery.
Clone: 10E10;
Host/Isotype: Mouse IgG2b;
Immunogen: Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI
Myeloma/fusion partners: SP2/0;
Specificity: Human PABP. Detects a band of approximately 70 kDa. It cross reacts with xenopus, rabbit & chicken. It does not react with mouse or drosophila.
Purification: Protein A;
Format: Purified antibody (from supernatant) containing PBS + 0.1% sodium azide;
Applications: E; IF; IP; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration
Relevance: The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. Grange et al. (1987) isolated a melanoma cell cDNA encoding human PABP. The predicted 633-amino acid protein contains 4 repeats of an approximately 80-amino acid unit in its N-terminal half. The authors found that this repeat region is highly conserved between human and yeast PABP and is sufficient for poly(A) binding. In vitro translation of the human PABP cDNA yielded a protein with an apparent molecular mass of 73 kD by SDS-PAGE. Northern blot analysis indicated that PABP is expressed as a 2.9-kb mRNA in human melanoma cells. Gorlach et al. (1994) noted that each of the 4 repeats of PABP is a ribonucleoprotein (RNP) consensus sequence RNA-binding domain. They determined that PABP has a pI of approximately 10.3 and is a very abundant, stable protein. Immunofluorescence studies of mammalian cells indicated that PABP is located exclusively in the cytoplasm. However, using both indirect immunofluorescence and tagging of PABP1 by fusion to the green fluorescent protein (GFP), Afonina et al. (1998) demonstrated that PABP1 shuttles between the nucleus and cytoplasm. PABP1 accumulated in the nucleus when transcription was inhibited, suggesting that active transcription is required for nuclear export of PABP1.
References: (click to view full details)
Expression of Kaposi's sarcoma-associated herpesvirus-encoded K10/10.1 protein in tissues and its interaction with poly(A)-binding protein - Takayuki Kanno et al ( Virology,15 August 2006)
Identification of the Junctional Plaque Protein Plakophilin 3 in Cytoplasmic Particles Containing RNA-binding Proteins and the Recruitment of Plakophilins 1 and 3 to Stress Granules- Ilse Hofmann et al ( Mol Biol Cell,March 2006)
Mammalian Smaug Is a Translational Repressor That Forms Cytoplasmic Foci Similar to Stress Granules- María V. Baez et al ( J. Biol. Chem,December 30, 2005)
The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity.- Görlach M ( Exp Cell Res.,April 1994)
Research areas: Chromatin; Dicer; P bodies/GW bodies; Regulation; RNAi;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
