The Nuclear Envelope, Signalling and the Nuclear Membrane – Lamins and Ageing- Good news emerging?
Studies have implicated Lamins in human disease for years. First in cancer, then in muscular dystrophy and cardiomyopathy, then ten years ago, in 2003 in premature ageing. Most recently, last year, Lamins have been implicated in normal ageing and Nuclear Antibodies against Lamins are now considered as universal markers of the ageing process. Lamins ( designated A,B and C ) … Continue reading
A cell-autonomous requirement for neutral sphingomyelinase 2 in bone mineralization
Abstract A deletion mutation called fro (fragilitas ossium) in the murine Smpd3 (sphingomyelin phosphodiesterase 3) gene leads to a severe skeletal dysplasia. Smpd3 encodes a neutral sphingomyelinase (nSMase2), which cleaves sphingomyelin to generate bioactive lipid metabolites. We examined endochondral ossification in embryonic day 15.5 fro/fro mouse embryos and observed impaired apoptosis of hypertrophic chondrocytes and severely undermineralized cortical bones in … Continue reading
Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly
Abstract During mitosis, the nuclear envelope merges with the endoplasmic reticulum (ER), and nuclear pore complexes are disassembled. In a current model for reassembly after mitosis, the nuclear envelope forms by a reshaping of ER tubules. For the assembly of pores, two major models have been proposed. In the insertion model, nuclear pore complexes are embedded in the nuclear envelope … Continue reading
Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly
Abstract During mitosis, the nuclear envelope merges with the endoplasmic reticulum (ER), and nuclear pore complexes are disassembled. In a current model for reassembly after mitosis, the nuclear envelope forms by a reshaping of ER tubules. For the assembly of pores, two major models have been proposed. In the insertion model, nuclear pore complexes are embedded in the nuclear envelope … Continue reading
igh-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine
Fairn et al. 194 (2): 257 Abstract Phosphatidylserine (PS) plays a central role in cell signaling and in the biosynthesis of other lipids. To date, however, the subcellular distribution and transmembrane topology of this crucial phospholipid remain ill-defined. We transfected cells with a GFP-tagged C2 domain of lactadherin to detect by light and electron microscopy PS exposed on the cytosolic … Continue reading
α-Synuclein and ALPS motifs are membrane curvature sensors whose contrasting chemistry mediates selective vesicle binding
Pranke et al. 194 (1): 89 Abstract Membrane curvature sensors have diverse structures and chemistries, suggesting that they might have the intrinsic capacity to discriminate between different types of vesicles in cells. In this paper, we compare the in vitro and in vivo membrane-binding properties of two curvature sensors that form very different amphipathic helices: the amphipathic lipid-packing sensor (ALPS) … Continue reading
POM121 and Sun1 play a role in early steps of interphase NPC assembly
Talamas and Hetzer 194 (1): 27 Abstract Nuclear pore complexes (NPCs) assemble at the end of mitosis during nuclear envelope (NE) reformation and into an intact NE as cells progress through interphase. Although recent studies have shown that NPC formation occurs by two different molecular mechanisms at two distinct cell cycle stages, little is known about the molecular players that … Continue reading
