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hnRNP A2B1 Antibody [DP3B3]

Staining of paraffin lung tissue sections showing squamous cell carcinoma, using hnRNP A2B1 antibody

Catalogue Number: IQ271

£183.00
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Target Antigen

hnRNP A2B1 

Primary Description

Mouse Anti-hnRNP A2B1 [DP3B3]

Catalogue Number

IQ271

Quantity

50ul 

Concentration

1mg/ml

Clone

DP3B3

Host

Mouse 

Isotype

IgG2a

Immunogen

Bacterially expressed His-hnRNPA2 fusion protein (Human)

 

Myeloma/fusion partners

SP2/0

Specificity

Reacts predominantly with a 36 kDa band (A2), a 38kDa band (B1)

Species Reactivity

Chicken, Human, Mouse, Rat, Pig

Purification

Protein A

Format

Purified antibody (from supernatant) containing PBS 0.1% sodium azide;

Applications

ICC/IF, IHC-Fr, ELISA, WB, IP, IHC-P

Dilutions

Optimal antibody dilution should be determined by titration, however as a guideline try; 

IHC-Fr: 1/100. PubMed: 17067748Fix with acetone

WB: 1/1500 - 1/6000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa)

IHC-P: Use a concentration of 1 µg/m

References

Rambaruth ND  et al. The lectin Helix pomatia agglutinin recognizes O-GlcNAc containing glycoproteins in human breast cancer. Glycobiology 22:839-48 (2012). WB, ICC/IF ; Human PUBMED

Wong PF et al. Eurycomanone suppresses expression of lung cancer cell tumor markers, prohibitin, annexin 1 and endoplasmic reticulum protein 28. Phytomedicine. 2012 Jan 15;19(2):138-44. doi: 10.1016/j.phymed.2011.07.001. Epub 2011 Sep 7. WB ; Human PMID: 21903368

Maeto CA  et al. Differential effect of acute and persistent Junin virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B. J Gen Virol 92:2181-90 (2011) PUBMED

Clower CV  et al. The alternative splicing repressors hnRNP A1/A2 and PTB influence pyruvate kinase isoform expression and cell metabolism. Proc Natl Acad Sci U S A 107:1894-9 (2010). WB ; Human PUBMED

Rosenberger S  et al. Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation. Proc Natl Acad Sci U S A : (2010). WB ; Human PUBMED

Santarosa M  et al. BRCA1 modulates the expression of hnRNPA2B1 and KHSRP. Cell Cycle 9:4666-4673 (2010). WB ; Human PUBMED

Nicholls CD et al. Multiple factors influence the normal and UV-inducible alternative splicing of PIG3. Biochim Biophys Acta. 2008 Dec;1779(12):838-49. doi: 10.1016/j.bbagrm.2008.08.009. Epub 2008 Aug 29. WB ; Human PMID: 18801469

Lee JH  et al. Alterations in Gemin5 expression contribute to alternative mRNA splicing patterns and tumor cell motility. Cancer Res 68:639-44 (2008). WB ; Human PUBMED

Honoré B et al. Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53. Proteome Sci. 2008 Jun 10;6:18. doi: 10.1186/1477-5956-6-18. WB ; Mouse PMID: 18544163

Katherine A. Peebles et al. Altered expression of splicing factor, heterogeneous nuclear ribonucleoprotein A2/B1, in mouse lung neoplasia. Molecular Carcinogenesis,3 May 2007 IHC WB ; Mouse PMID: 17477362

Inoue A et al. Loss-of-function screening by randomized intracellular antibodies: identification of hnRNP-K as a potential target for metastasis. Proc Natl Acad Sci U S A. 2007 May 22;104(21):8983-8. Epub 2007 May 4. WB ; Human PMID: 17483488

Griffith BN et al. Identification of hnRNPs K, L and A2/B1 as candidate proteins involved in the nutritional regulation of mRNA splicing. Biochim Biophys Acta. 2006 Nov-Dec;1759(11-12):552-61. Epub 2006 Oct 6. WB ; Mouse PMID: 17095106 

Szeszel-Fedorowicz W et al. An exonic splicing silencer is involved in the regulated splicing of glucose 6-phosphate dehydrogenase mRNA. J Biol Chem. 2006 Nov 10;281(45):34146-58. Epub 2006 Sep 15. WB ; Human Rat PMID: 16980303

Strom A  et al. Identification of prion protein binding proteins by combined use of far-Western immunoblotting, two dimensional gel electrophoresis and mass spectrometry. Proteomics 6:26-34 (2006) PUBMED

Zech VF  et al. Prognostic and diagnostic relevance of hnRNP A2/B1, hnRNP B1 and S100 A2 in non-small cell lung cancer. Cancer Detect Prev 30:395-402 (2006). IHC-Fr ; Human PUBMED

Iwahashi CK et al. Protein composition of the intranuclear inclusions of FXTAS. Brain. 2006 Jan;129(Pt 1):256-71. Epub 2005 Oct 24. WB ICC/IF ; Human PMID: 16246864

Xia H Regulation of gamma-fibrinogen chain expression by heterogeneous nuclear ribonucleoprotein A1. J Biol Chem 280:13171-8 (2005) PUBMED

Hong OK  et al. Proteomic analysis of differential protein expression in response to epidermal growth factor in neonatal porcine pancreatic cell monolayers. J Cell Biochem 95:769-81 (2005) PUBMED

Molecular characterization of the hnRNP A2/B1 proteins: tissuespecific expression and novel isoforms. Kamma H ( Exp Cell Res.,Feb 1999) PUBMED

Database links

Entrez Gene: 3181 Human

Omim: 600124 Human

SwissProt: O88569 Human

SwissProt: P22626 Human

Unigene: 232400 Human

Unigene: 487774 Human

Research areas

RNA Processing;

Also known as

Heterogeneous nuclear ribonucleoprotein A2 antibody;Heterogeneous nuclear ribonucleoprotein A2/B1antibody;Heterogeneous nuclear ribonucleoprotein B1 antibody;Heterogeneous nuclear ribonucleoproteins A2/B1antibody;hnRNP A2 antibody;hnRNP A2 / hnRNP B1 antibody;hnRNP B1 antibody;hnRNP-A2 antibody;hnRNP-B1antibody;hnRNPA2 antibody;hnRNPB1 antibody;HNRPA2 antibody;HNRPA2B1 antibody;HNRPB1 antibody;Nuclearribonucleoprotein particle A2 protein antibody;RNP A2 antibody;RNP B1 antibody;RNP-A2 antibody;RNP-B1antibody;RNPA2 antibody;RNPB1 antibody;SNRPB1 antibody


THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC USE.

Print the Datasheet for hnRNP A2B1 Antibody [DP3B3]

  • This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA) These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm The hnRNP proteins have distinct nucleic acid binding properties The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms

Immunofluorescence protocol - Formaldehyde fixation

   1. Collect cells from T.c.unit and remove media from petri dish using suction.
   2. Wash with 1x PBS and remove.
   3. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperature on an orbital shaker.
   4. Remove PFA and incubate in 0.5% Triton X-IOO in 1x PBS for 5 minutes at room temperature.
   5. Prepare blocking reagent, this is also the antibody diluent.
   6. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker.
   7. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature.
   8. Prepare primary antibodies (50?l/coverslip) and moist staining chambers.
   9. Wash cells 2x with lx PBS at room temperature and air dry briefly.
  10. Incubate with primary antibody for 1 hr at room temperature in the dark in staining chambers. During this time prepare the secondary antibody.
  11. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)
  12. Incubate with secondary antibody for 1 hour at room temperature in the dark in staining chambers.
  13. Wash cells 5x with 1x PBS.
  14. Mount in Dapi.

Solutions (prepare fresh the same day of staining).

    * 1x Phosphate buffered saline.
    * Blocking reagent: 1% NCS in 1x PBS (use fresh l0x PBS).
    * Fixation solution: 3.5% Para formaldehyde.

1.75g PFA in 20 ml d.H20 plus 5 drops 1M NaOH. Stir on a hot plate at 50-60°C until dissolved. Add 4 drops IN HCI and check pH indicator strip. PH should be 7.4. Complete volume with d.H20 to 25ml and add 25ml 2xPBS. Check pH before adding to cover slips.


Immunofluorescence protocol - Methanol/acetone fixation

   1. Collect cells from T.C.unit and remove media from petri dish using suction.
   2. Wash with 1x PBS and remove.
   3. Fix cells with cold methanol: acetone 1: 1 for 10 minutes on ice.
   4. Prepare blocking reagent, this is also the diluent for the antibodies.
   5. Remove fixative and wash cells 3x with Ix PBS at RT, for 4 minutes/wash on orbital shaker.
   6. Block with 1% NCS and Ix PBS for 30 minutes at RT.
   7. Prepare primary antibodies (50?l/coverslip) and moist staining chambers.
   8. Wash cells 2x with 1 x PBS at RT and air dry for approximately 7 minutes.
   9. Incubate with primary antibody for 1 hr at RT in the dark in staining chambers.  During this time prepare secondary antibody.
  10. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)
  11. Incubate with secondary antibody for 1 hr at R T in the dark in staining chambers.
  12. Wash cells 5x with 1x PBS.
  13. Mount in Dapi.

Solutions (prepare fresh the same day of staining)

    * 1x Phosphate buffered saline.
    * Blocking reagent: 1% NCS in 1x PBS (use fresh 10x PBS).
    * Fixation solution: methanol:acetone 1: 1 ice cold.



Western Blotting Protocol

   1. Transfer gel to PDVF or nitrocellulose membrane
   2. Place membrane in plastic tray in blocking buffer for one hour with agitation
   3. Rinse in wash buffer
   4. Incubate in wash buffer plus primary antibody for one hour
   5. Wash 6 X 5 minutes with wash buffer
   6. Incubate in wash buffer plus secondary antibody for one hour
   7. Wash 6X 5 minutes with wash buffer
   8. Detect (e.g. ECL, Amersham according to manufacturers instructions)

Wash buffer
PBS + 0.1% Tween 20

Blocking buffer
Wash buffer + 5% dried milk powder

The concentration of antibodies used depends on each antibody, the amount of antigen and the detection method used. Generally, dilution is in the range of a few hundred times dilution to a few thousand times dilution, but usually has to be determined empirically.

Please click a question to be shown the answer, if you cannot find what you are looking for please contact us.

How do I place an order?
How long does delivery take?
Do you guarantee your antibodies?
Do you charge VAT?
What if I don't have a purchase order number?


How do I place an order?

When placing an order we require a purchase order number, plus name and contact details of the purchaser, and the person who will be using the product. We will add VAT for every order received from an EU country, unless you are VAT exempt. For such VAT exempt customers we will need to receive a copy of the exemption certificate with the order.

Orders can be placed either by our website, via email or by mail. Please see our contact us page for more details

All orders are subject to availability. Prices of products do not include shipping, VAT or import duties, where these are applicable.
Price and other information provided are subject to change without notice. While every effort is made to keep information provided Up to date, ImmuQuest will not be liable if errors should occur in such information.

Formal acceptance of an order will take place when the goods are dispatched. If prices should be changed between the time of receipt of an order and dispatch, ImmuQuest will contact you in advance.


Ordering via the website

To order via the website you will need to find the product(s) you are looking for by either using our search, browsing our categories or using our full antibody catalogue.

Once you have found the antibodies you are looking for you can view more detailed information about a specific antibody by clicking the product or the 'View' button. Once you are sure it is the antibody you are looking for you can add it to your order by clicking the 'Add To Order' button.

This places the antibody in your order basket, you can add as many antibodies as you wish. Once you have selected all of the antibodies you wish to order then click the checkout button. If you are not logged in you will be asked to login or to register, if you are logged in you will be taken to the checkout page.

On the checkout page you are able to double check your order and enter a purchase order number if you have one.

Once you are happy your order is correct you can purchase the order with debit/credit card details and your order will be submitted. Once we have received and checked your order we will contact you to arrange payment and delivery.


 
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How long does delivery take?

Delivery usually takes no more than one week from reciept of an order.

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Do you guarantee your antibodies?

Yes. Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.

If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.

Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.

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Do you charge VAT?

If you are in the EU and you are no exempt from paying VAT then we must charge VAT (17.5%).

Customers that are exempt need to fax an exemption form showing their vat exemption number.

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What if I don't have a purchase order number?

You can still place an order without providing a purchase order number but we must recieve payment before your order is despatched.

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Non-website orders

Non-website orders can be placed directly with Immuquest at sales@immuquest.com or click on the "contact us" tab for address details

 

Product Guarantee

We are strongly committed to providing the best quality products, and the best service possible. In order to do this we depend on your feedback.

Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.

If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.

Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.

If you have any questions please contact us using the following details:
ImmuQuest Ltd
26 The Green
Seamer
North Yorkshire
TS9 5LP
UK

Tel: 01642 713533
Fax: 01642 713988
International Tel: +44 1642 713533
International Fax: +44 1642 713988

When placing an order ImmuQuest require a purchase order number, plus name and contact details of the purchaser, and the person who will be using the product. ImmuQuest will also need a VAT number for customers in the European Union.UK customers that are VAT exempt need to fax an exemption certificate.

Orders can be placed either by our website, via email or by mail. Please see our contact us page for more details. All orders are subject to availability. Prices of products do not include shipping, VAT or import duties where these are applicable.

Price and other information provided are subject to change without notice. While every effort is made to keep information provided Up to date, ImmuQuest will not be liable if errors should occur in such information. Formal acceptance of an order will take place when the goods are dispatched. If prices should be changed between the time of receipt of an order and dispatch, ImmuQuest will contact you in advance.

Research Areas
RNA Processing
Research
946-Catenin Interacts With the FUS Proto-oncogene Product and Regulates Pre-mRNA Splicing - Satoshi Sato et al Altered expression of splicing factor heterogeneous nuclear ribonucleoprotein A2/B1 in mouse lung neoplasia - Katherine A Peebles et al Molecular characterization of the hnRNP A2/B1 proteins tissue-specific expression and novel isoforms - Kamma H Protein composition of the intranuclear inclusions of FXTAS - C K Iwahashi et al

Offline Orders

For offline orders please call +44 (0) 1642 713533 or click here to email us.

Delivery & returns

We use federal Express for UK & international shipments, andfor standard shipments the cost is £30 UK & £55 international. If the customer uses their account, we charge a £20 handling fee (UK & international)

We are strongly committed to providing the best quality products, and the best service possible.

Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.

If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.

Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.

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