Microtubules are key components of the cell infrastructure or cytoskeleton. These polymers are important inter alia in maintaining cell structure, facilitating intracellular transportation and spindle formation in mitosis. Many proteins bind to the microtubule including motor proteins, severing proteins and proteins important for managing microtubule dynamic mechanisms.
Microtublues are organised by the MTOC’s, microtubule organising centres and areconstructed of tublin a rope like polymer.
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Aurora B Antibody - Sheep Anti- Aurora B 0.2ml
Aurora B is a mitotic protein kinase that phosphorylates histone H3 (probably on Serine 10) behaves as a chromosomal passenger protein and may regulate several stages of mitosis such as centrosome separation chromosome segregation and cytokinesis It localizes to the inner centromere region from prophase to anaphase
£226.00
CENP-E Antibody - Mouse Anti-CENP-E 0.1ml
CENPE is a 250-300 kDa human centromere-associated kinesin-like motor protein that accumulates in G2 phase In contrast to other centromere proteins CENPE is not detected at centromeres during interphase and first appears at the centromere region of chromosomes during prometaphase CENPE function is required for the transition from metaphase to anaphase CENPE is probably one of the motors responsible for mammalian chromosome movement and/or spindle elongation
£226.00
CENP-E Antibody - Mouse Anti-CENP-E 50ul
CENPE is a 250-300 kDa human centromere-associated kinesin-like motor protein that accumulates in G2 phase In contrast to other centromere proteins CENPE is not detected at centromeres during interphase and first appears at the centromere region of chromosomes during prometaphase CENPE function is required for the transition from metaphase to anaphase CENPE is probably one of the motors responsible for mammalian chromosome movement and/or spindle elongation
£183.00
Mad1p Antibody- Rabbit Anti-Mad1p
MAD1 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad1p becomes hyperphosphorylated upon spindle depolymerization
£226.00
Mad2L1 Antibody- Mouse Anti-Mad2L1
Mad2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore spindle attachment and delays the onset of anaphase when this process is not complete It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate
£226.00
Mad2p Antibody- Rabbit Anti-Mad2p
MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators.hMAD2 is the human homologue of yeast MAD2
£226.00
NEK2 Antibody- Sheep Anti-NEK2
NEK2 is closely related in its catalytic domain to the serine/threonineprotein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers
£226.00
Nesprin 1 Antibody- Rabbit Anti-Human Nesprin 1
There are two genes encoding members of a new family of type II integral membrane proteins Both are ubiquitously expressed and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein with the smaller isoforms being truncated at the N-terminusThese proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat as they are characterized by the presence of multiple clustered spectrin repeats bipartite nuclear localization sequences and a conserved C-terminal single transmembrane domain Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal TM domain-containing sequence essential for perinuclear localization Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation Nesprins may function as dystrophins of the nucleus to maintain nuclear organization and structural integrity There are two genes encoding members of a new family of type II integral membrane proteins Both are ubiquitously expressed and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein with the smaller isoforms being truncated at the N-terminusThese proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat as they are characterized by the presence of multiple clustered spectrin repeats bipartite nuclear localization sequences and a conserved C-terminal single transmembrane domain Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal TM-domain-containing sequence essential for perinuclear localization Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation Nesprins may function as dystrophins of the nucleus to maintain nuclear organization and structural integrity
£226.00
TRF1 Antibody- Mouse Anti-TRF1
The TRF1 gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex It is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase acting in cis to limit the elongation of individual chromosome ends The protein structure contains a C-terminal Myb motif a dimerization domain near its N-terminus and an acidic N-terminus Two transcripts of this gene are alternatively spliced products
£183.00
