Nuclear transport antibodies relate to the large molecules are carried bi-directionally across the nuclear envelope by a RAN (ras-related nuclear protein) GTP nuclear transport cycle.
Nuclear transport processes RNA and proteins are enabled to enter the nucleus via specialist karyopherin proteins importins and facilitated outbound by exportins via the nuclear pore structures e.g. Transportin1 . Both exportins and importins abilities are regulated by GTPase.
Nuclear transport antibodies are useful in understanding the direction of the nuclear transport cycle is triggered by localisation signals in an active process that can be negatively affected in human neurodegenerative diseases like Parkinsons and suggest nuclear protein import directly participate in the pathogenesis of diseases such as hypertension, atherosclerosis, cancer, viral infection, and diabetes.
Nuclear transport antibodies are available in the following various quantities including 0.05 ml, 0.1ml, 0.2 ml, 1 ml
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Emerin Antibody- Sheep Anti-Emerin
Emerin is a serine rich nuclear membrane protein and a member of the nuclear lamina associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene
£226.00
Karyopherin alpha2 (KPNA2) Antibody- Rat Anti-Karyopherin alpha2 (KPNA2)
The import of proteins into the nucleus is a process that involves at least 2 steps The first is an energy independent docking of the protein to the nuclear envelope and the second is an energy dependent translocation through the nuclear pore complex Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart Proteins involved in the first step of nuclear import have been identified in different systems These include the Xenopus protein importin and its yeast homolog SRP1 (a suppressor of certain temperature sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae) which bind to the NLS KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins KPNA2 also may play a role in V(D)J recombination
£226.00
Ran Antibody- Rabbit Anti-Ran
Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex The Ran protein is also involved in control of DNA synthesis and cell cycle progression Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1) Mutations in Ran disrupt DNA synthesis Because of its many functions it is likely that Ran interacts with several other proteins Ran regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules Ran could be a key signaling molecule regulating microtubule polymerization during mitosis RCC1 generates a high local concentration of Ran-GTP around chromatin which in turn induces the local nucleation of microtubules Ran is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor Polyglutamine repeat expansion in the AR is linked to Kennedys disease (X-linked spinal and bulbar muscular atrophy) Ran coactivation of the AR diminishes with polyglutamine expansion within the AR and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedys disease
£183.00
RanGAP Antibody- Rabbit Anti-RanGAP
Ran is a small signaling GTPase that is involved in nucleocytoplasmic
transport. Two additional functions of animal Ran in the formation of spindle
asters and the reassembly of the nuclear envelope in mitotic cells have been
recently reported. In contrast to Ras or Rho, Ran is not associated with
membranes. Instead, the spatial sequestering of its accessory proteins, the Ran
GTPase-activating protein RanGAP and the nucleotide exchange factor RCC1,
appears to define the local concentration of RanGTP vs. RanGDP involved in
signaling. Mammalian RanGAP is bound to the nuclear pore by a mechanism
involving the attachment of small ubiquitin-related modifier protein (SUMO)
to its C terminus and the subsequent binding of the SUMOylated domain to the
nucleoporin Nup358
£226.00
RanGAPAntibody - Rabbit Anti-RanGAP
Ran is a small signaling GTPase that is involved in nucleocytoplasmic
transport. Two additional functions of animal Ran in the formation of spindle
asters and the reassembly of the nuclear envelope in mitotic cells have been
recently reported. In contrast to Ras or Rho, Ran is not associated with
membranes. Instead, the spatial sequestering of its accessory proteins, the Ran
GTPase-activating protein RanGAP and the nucleotide exchange factor RCC1,
appears to define the local concentration of RanGTP vs. RanGDP involved in
signaling. Mammalian RanGAP is bound to the nuclear pore by a mechanism
involving the attachment of small ubiquitin-related modifier protein (SUMO)
to its C terminus and the subsequent binding of the SUMOylated domain to the
nucleoporin Nup358
£183.00
RanGEF (RCC1) Antibody- Rabbit Anti-RanGEF (RCC1)
Ran GTPase plays important roles in nucleocytoplasmic transport in interphase and in both spindle formation and nuclear envelope (NE) assembly during mitosis The latter functions rely on the presence of high local concentrations of GTP bound Ran near mitotic chromatin RanGTP localization has been proposed to result from the association of Rans GDP/GTP exchange factor RCC1 with chromatin but Ran is shown here to bind directly to chromatin in two modes either dependent or independent of RCC1 and where bound to increase the affinity of chromatin for NE membranes
£183.00
TAP Antibody- Mouse Anti-TAP (tip associating protein)
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters ABC proteins transport various molecules across extra- and intra-cellular membranes ABC genes are divided into seven distinct subfamilies (ABC1 MDR/TAP MRP ALD OABP GCN20 White) This protein is a member of the MDR/TAP subfamily Members of the MDR/TAP subfamily are involved in multidrug resistance The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble Mutations in this gene may be associated with ankylosing spondylitis insulin-dependent diabetes mellitus and celiac disease
£226.00
Transportin1 (TRN) Antibody- Mouse Anti-Transportin1
Heterogeneous nuclear ribonucleoprotein hnRNPA1 is an abundant nuclear protein that plays an important role in pre-mRNA processing and mRNA export from the nucleus A1 shuttles rapidly between the nucleus and the cytoplasm and a 38-amino acid domain M9 serves as the bi-directional transport signal of A1 Recently a 90-kD protein transportin was identified as the mediator of A1 nuclear import Transportin mediates the nuclear import of additional hnRNP proteins including hnRNPF The nuclear localization of A1 is dependent on ongoing RNA polymerase II transcription
£226.00
