GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells The antibody may be used to track the purification of proteins expressed in bacteria yeast or insect cells In Western blotting of bacterial extracts clone ADII10 has been shown not to cross react with any endogenous products although some cross reactivity may be seen with extracts of insect or mammalian cells